LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1649..1745)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1760..1779)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1787..1803
                     /note="M13 Reverse priming site"
     gene            1916..2725
                     /note="Kanamycin resistance gene"
     rep_origin      2846..3519
                     /note="pUC origin"
     vector          join(1657..3522,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1642
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:61575 IMAGE:6501263"
                     /tissue_type="Uterus, leiomyosarcoma"
                     /clone_lib="NIH_MGC_71"
                     /lab_host="DH10B"
                     /note="Vector: pCMV-SPORT6"
     gene            692..1642
                     /gene="CHST14"
                     /gene_synonym=D4ST-1
                     /gene_synonym=HD4ST
                     /gene_synonym=HNK1ST
                     /db_xref="GeneID:113189"
                     /db_xref="HGNC:24464"
     CDS             692..1642
                     /dnas_title="carbohydrate (N-acetylgalactosamine 4-0)
                     sulfotransferase 14"
                     /gene="CHST14"
                     /gene_synonym=D4ST-1
                     /gene_synonym=HD4ST
                     /gene_synonym=HNK1ST
                     /codon_start=1
                     /product="carbohydrate (N-acetylgalactosamine 4-0)
                     sulfotransferase 14"
                     /protein_id="AAH53633.1"
                     /db_xref="GI:31565498"
                     /db_xref="GeneID:113189"
                     /db_xref="HGNC:24464"
                     /translation="MFPRPLTPLAAPNGAEPLGRALRRAPLGRARAGLGGPPLLLPSM
                     LMFAVIVASSGLLLMIERGILAEMKPLPLHPPGREGTAWRGKAPKPGGLSLRAGDADL
                     QVRQDVRNRTLRAVCGQPGMPRDPWDLPVGQRRTLLRHILVSDRYRFLYCYVPKVACS
                     NWKRVMKVLAGVLDSVDVRLKMDHRSDLVFLADLRPEEIRYRLQHYFKFLFVREPLER
                     LLSAYRNKFGEIREYQQRYGAEIVRRYRAGAGPSPAGDDVTFPEFLRYLVDEDPERMN
                     EHWMPVYHLCQPCAVHYDFVGSYERLEADANQVLEWVRAPPHVRFPARQAWYRPASPE
                     SLHYHLCSAPRALLQDVLPKYILDFSLFAYPLPNVTKEACQQ"
     misc_feature    692..1642
                     /note="D4ST1 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cgagcggggc atcctggccg agatgaagcc
        721 cctgcccctg cacccgcccg gccgcgaggg cacagcctgg cgcgggaaag cccccaagcc
        781 tgggggcctg tccctcaggg ctggggacgc ggacttgcaa gtgcggcagg acgtccggaa
        841 caggaccctg cgggcggtgt gcggacagcc aggcatgccc cgggacccct gggacttgcc
        901 ggtggggcag cggcgcaccc tgctgcgcca catcctcgta agtgaccgtt accgcttcct
        961 ctactgctac gtccccaagg tggcctgctc taactggaag cgggtgatga aggtgctggc
       1021 aggcgtcctg gacagcgtgg acgtccgcct caagatggac caccgcagtg acctggtgtt
       1081 cctggccgac ctgcggcctg aggagattcg ctaccgcctg cagcactact ttaagttcct
       1141 gtttgtgcgg gagcccttgg aacgcctcct ctctgcctac cgcaacaagt ttggcgagat
       1201 ccgagagtac cagcaacgct atggggctga gatagtgagg cggtacaggg ctggagcggg
       1261 gcccagccct gcaggcgacg atgtcacatt ccccgagttc ctgagatacc tggtggatga
       1321 ggaccctgag cgcatgaatg agcattggat gcccgtgtac cacctgtgcc agccttgtgc
       1381 cgtgcactat gactttgtgg gctcctatga gaggctggag gctgatgcaa atcaggtgct
       1441 ggagtgggta cgggcaccac ctcacgtccg atttccagct cgccaggcct ggtaccggcc
       1501 agccagcccc gaaagcctgc attaccactt gtgcagtgcc ccccgggccc tgctgcagga
       1561 tgtgctgcct aagtatatcc tggacttctc cctctttgcc tacccactgc ctaatgtcac
       1621 caaggaggcg tgtcagcagt gaTAGGACCC AGCTTTCTTG TACAAAGTTG GCATTATAAG
       1681 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT
       1741 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC
       1801 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT
       1861 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG
       1921 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA
       1981 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA
       2041 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA
       2101 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT
       2161 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA
       2221 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT
       2281 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA
       2341 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG
       2401 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA
       2461 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT
       2521 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA
       2581 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA
       2641 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA
       2701 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG
       2761 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA
       2821 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
       2881 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
       2941 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
       3001 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
       3061 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
       3121 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
       3181 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
       3241 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
       3301 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
       3361 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
       3421 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
       3481 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT
//